NEGEK0006 Kanamycin (KA) ELISA Kit
This Kit is for the quantitative Measurement of Kanamycin Residues in Samples such as Milk, Milk Powder, Tissue samples(Chicken, Pork)
Specifications of Kanamycin (KA) ELISA Kit
Product Information | |
Cat. NO. | NEGEK0006 |
Products Name | Kanamycin (KA) ELISA Kit |
Species | General |
Product Size | 96 Tests |
Concentration | 0-32ng/mL |
Sensitivity | 0.5ng/mL(ppb) |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | ELISA Kit for the quantitative Measurement of Kanamycin Residues in Samples such as Milk, Milk Powder, Tissue samples(Chicken, Pork) |
Assay Time | 3 hours and 15 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
| Kanamycin Standard (Stock Solution) -3.2μg/mL | 20ul | 1 vial |
| Kanamycin Monoclonal Antibody (100×) | 100μL | 1 vial |
| Biotin-labeled Detection Antibody (100×) | 150μL | 1 vial |
| Streptavidin-HRP Conjugate (150×) | 100μL | 1 vial |
| Diluent Buffer (10×) | 10mL | 1 vial (Avoid Light) |
| TMB Substrates | 10ml | 1 vial |
| Wash Solution (100×) | 10ml | 1 vial |
Stop Solution | 10ml | 1 vial |
| Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit employs an indirect competitive ELISA method. The microplate is pre-coated with kanamycin, and standards (or samples) along with kanamycin monoclonal antibodies are added simultaneously. The coated antigen on the microplate competes with the kanamycin in the standards (or samples) for binding to the monoclonal antibody. After incubation at room temperature, unbound components are washed away. A biotin-labeled secondary antibody is then added to bind to the kanamycin monoclonal antibody. Following another incubation and wash step, Streptavidin-HRP Conjugate is added. Finally, the reaction is developed using TMB substrate, and the absorbance of the sample is inversely correlated with the kanamycin concentration. The kanamycin concentration in the sample is determined by comparing the absorbance to the standard curve and multiplying by the corresponding dilution factor. |
Quality Control on Kanamycin (KA) ELISA Kit
Coefficient of Variance | Intra Variation:4-10.19% | ||
Inter Variation: 7.68-9.96% | |||
| Recovery | Sample Type | Recovery Rate | |
| Milk | 80-120% | ||
| Milk Powder | 80-100% | ||
| Tissues | 80-120% | ||
Specificity/ Cross-reactivity | Sample | Cross-Reactivity | |
TOB (Tobramycin) | 37.79% | ||
NEO (Neomycin) | 0.02% | ||
STR (Streptomycin) | 0.01% | ||
GM (Gentamicin) | 0.08% | ||
BlueGene Biotech Product Show
Summary of the Assay Procedure for Kanamycin (KA) ELISA Kit
Citations of Kanamycin (KA) ELISA Kit
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