Name: Protein L (PL) ELISA Kit(NEGEP1270)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Protein L (PL) ELISA Kit(NEGEP1270)

Immunology_copy20250611

NEGEP1270 Protein L (PL) ELISA Kit

This Kit is for the quantitative Measurement of Protein L Residues in Protein Purification Process, and End-Product.

Products

Specifications of NEGEP1270 Protein L (PL) ELISA Kit

Product Information
Cat. NO.	NEGEP1270
Products Name	Protein L (PL) ELISA Kit
Species	General
Product Size	96 Tests
Concentration	0.5-32ng/mL
Sensitivity	LOD: 0.125ng/mL; LOQ: 0.5ng/mL
Principal	Sandwich ELISA
Sample Volume	100 ul
Sample Type	ELISA Kit for the quantitative Measurement of Protein L Residues in Protein Purification Process, and End-Product
Assay Time	3 hours and 15 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
Reagents	Specification	Quantity
Pre-Coated Microplate (Detachable)	96 wells	1 plate (Keep Sealed)
Standard (Stock Solution) -16ug/ml	10ul	1 vial
Detection Antibody Concentrate (100×)	150μL	1 vial
HRP-conjugated Concentrate (150×)	100μL	1 vial
Sample Pretreatment Solution	5mL	1 vial
TMB Substrates	10ml	1 vial (Avoid Light)
Stop Solution	10ml	1 vial
Wash Solution (100×)	10ml	1 vial
Diluent Buffer (10×)	10ml	1 vial
Detection Antibody Diluent (1×)	10mL	2 vials
Plate Sealer		4 pieces
Instruction Manual		1

Principle of the Assay

This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of protein L in samples. Before starting the ELISA experiment, mix the samples and serially diluted standards with denaturing solution, and incubate at room temperature to completely dissociate Protein L from the antibodies. After pretreatment, add the detection antibody to the microplate wells pre-coated with chicken anti-Protein L antibody. Then add the denatured standards and samples. Incubate at room temperature to allow the specific binding of antigen-antibody, capturing Protein L from the samples and standards onto the microplate. After incubation, a sandwich complex forms with the coating antibody, Protein L, and detection antibody. Wash the plate to remove unbound substances. Add streptavidin-labeled HRP and incubate at room temperature. After washing the plate again, add TMB substrate solution. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total protein L bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of protein L in samples is then determined by comparing the O.D. of the samples to the standard curve.