SC-D050T(50T) Saccharomyces Cerevisiae Host Cell DNA Residue Detection Kit
The Saccharomyces Cerevisiae Host Cell DNA Residue Detection Kit can be used for Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from host cell.
Introduction |
This kit utilizes the TaqMan probe-based fluorescence quantitative PCR (qPCR) method. By employing highly specific primers and probes, it offers exceptional specificity and sensitivity, with a limit of quantification (LOQ) as low as 3fg/μL. The kit is fully compatible with our Magnetic Residual DNA Sample Preparation Kit (Cat# CG-DP050 or CG-DP100), enabling seamless integration into your workflow. The preparation process of DNA Control is completely consistent with that of the National Standard reference of host cell DNA; therefore, it has high purity and no protein or ion interference. DNA Control has been calibrated by National Standard to ensure the accuracy of the sample quantitative detection. The kit provides DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments. |
| Kit Components | ||
DNA Amplification | ||
Components NO. | Components Name | Cat#/Size CK-D050T(50T) |
B1 | 2XqPCR Mix | 0.625mL |
B2 | Primer&Probe Mix | 100μL |
B3 | 2×1.5mL | |
B4 | DNA Control (30ng/μL) | 25μL |
B5 | RNase-Free H2O | 0.5mL |
B6 | 50X ROX Reference Dye(Optional) | 0.15ml |
Shipping and Storage | |
| 1 | All components are shipped on dry ice. |
| 2 | The kit should be stored at -20℃ and it is recommended to be used within one year. The component of B2 should be stored protected from light. |
Components | Volume(μL) |
2XqPCR Mix | 12.5 |
Primer&Probe Mix | 2 |
DNA template (control or sample) | 5 |
Add water | 5.5 |
Total Volume | 25 |
Mix solution = (number of reaction wells+4) * (12.5+2+5.5) μL (including the volume lost in the 4 wells).
The detection range of the standard curve mentioned above is suitable for most experiments and can be adjusted as needed, such as 30fg/μL to 300pg/μL.
Standard Curve: R²> 0.99; Amplification Efficiency: 90%≤Eff%≤110%; Slope: -3.8~-3.1.
The recovery rate of spiked samples=(measured value of spiked samples - measured value of samples)/theoretical value of spiked samples * 100%, with a range of 50% -150%.
No Template Control (NTC): In the reaction system, replacing the target template with DNA Dilution Buffer while keeping other components unchanged, and the Ct value obtained should be 'Undetermined' or Ct value≥35.
Accuracy | Intra Variation <15% |
Inter Variation <15% | |
Recovery | 54-90% |
| Limit of Quantitation | 3fg/μL |
Specificity | |
| Host | Result |
| HEK293 | No cross-reactivity |
| CHO | No cross-reactivity |
| E.coli | No cross-reactivity |
| Vero | No cross-reactivity |
| Pichia Pastoris | 0.018% |
| NS1 | No cross-reactivity |
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