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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

E08N0010 Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

The Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit can be used to identify samples from the Canine species. Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox can also be called MOX1, GP91-2, NOH1, NADPH Oxidase 1, Mitogenic Oxidase(Pyridine Nucleotide-Dependent Superoxide-Generating, NADH/NADPH mitogenic oxidase subunit P65-MOX, NADHPox.

Specifications of Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

Product Information

Cat. No.

E08N0010

Product Name

Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NADHPox ELISA kit uses an anti-NADHPox antibody and an NADHPox-HRP conjugate in a competitive enzyme immunoassay method. NADHPox-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NADHPox antibody binding site between NADHPox from samples and NADHPox-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NADHPox. Since the number of sites is limited, as more sites are occupied by NADHPox from the sample, fewer sites are left to bind NADHPox-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NADHPox concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NADHPox and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

Summary of the Assay Procedure for Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

Citations of Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA kit

E08N0010 has been referenced in the below publications:

Investigation of the mechanism underlying the antihypertensive effect of catheter-based radiofrequency renal sympathetic denervation in hypertensive dogs.

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