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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Aquaporin 1 ELISA kit

E08A0863 Canine Aquaporin 1 ELISA kit

Canine Aquaporin 1 ELISA kit is suitable for the detection of samples from Canine species. Aquaporin 1 can also be called AQP-CHIP, CHIP28, CO, channel-forming integral protein 28, WCH-CD, AQP1a, AQP1b, AQP1, AQP-1.

Specifications of Canine Aquaporin 1 ELISA kit

Product Information

Cat. No.

E08A0863

Product Name

Canine Aquaporin 1 ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AQP 1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AQP 1 antibody and an AQP 1-HRP conjugate. The assay sample and buffer are incubated together with AQP 1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AQP 1 concentration since AQP 1 from samples and AQP 1-HRP conjugate compete for the anti-AQP 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AQP 1 from the sample, fewer sites are left to bind AQP 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Aquaporin 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

85-106

1:4

86-108

1:8

81-111

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AQP 1 and analogues was observed.


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Summary of the Assay Procedure for Canine Aquaporin 1 ELISA kit

Summary of the Assay Procedure for Canine Aquaporin 1 ELISA kit

Citations of Canine Aquaporin 1 ELISA kit

E08A0863 has been referenced in the below publications:

Increased CSF aquaporin-4,and interleukin-6 levels in dogs with idiopathic communicating internal hydrocephalus and a decrease after ventriculo-peritoneal shunting.

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