E08M0329 Canine Matrix metalloproteinase 9 ELISA kit
Canine Matrix metalloproteinase 9 ELISA kit is suitable for the detection of samples from Canine species. Matrix metalloproteinase 9 can also be called Matrix metalloproteinase-9 precursor, 92 kDa type IV. Collagenase, 92 kDa gelatinase, Gelatinase B, GELB, MMP 9, MMP9, MMP-9.
Product Information | |
Cat. No. | E08M0329 |
Product Name | Canine Matrix metalloproteinase 9 ELISA kit |
Species | Canine |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MMP 9 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MMP 9. Standards or samples are then added to the microtiter plate wells and MMP 9 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MMP 9 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MMP 9 are added to each well to “sandwich” the MMP 9 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MMP 9 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP 9 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 86-115% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-114 | |
1:4 | 87-117 | |
1:8 | 80-112 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MMP 9 and analogues was observed. | |
E08M0329 has been referenced in the below publications:
Metoprolol inhibits profibrotic remodeling of epicardial adipose tissue in a canine model of chronic obstructive sleep apnea.
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