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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Endothelin 1 ELISA kit

E08E0040 Canine Endothelin 1 ELISA kit

Canine Endothelin 1 ELISA kit is suitable for the detection of samples from Canine species. Endothelin 1 can also be called Endothelin-1, Big endothelin 1, EDN1 protein, Endothelin1, ET1, PPET1, Preproendothelin 1.

Specifications of Canine Endothelin 1 ELISA kit

Product Information

Cat. No.

E08E0040

Product Name

Canine Endothelin 1 ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

EDN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EDN1 antibody and an EDN1-HRP conjugate. The assay sample and buffer are incubated together with EDN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EDN1 concentration since EDN1 from samples and EDN1-HRP conjugate compete for the anti-EDN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by EDN1 from the sample, fewer sites are left to bind EDN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EDN1 concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Endothelin 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-108%

Linearity

Diluent Ratio

Range %

1:2

86-105

1:4

85-110

1:8

82-112

Specificity/Cross-reactivity

No significant cross-reactivity or interference between EDN1 and analogues was observed.


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Summary of the Assay Procedure for Canine Endothelin 1 ELISA kit

Summary of the Assay Procedure for Canine Endothelin 1 ELISA kit

Citations of Canine Endothelin 1 ELISA kit

E08E0040 has been referenced in the below publications:

Effects of bone marrow mesenchymal stem cells and benefiting-Qi nourishing-Yin and dissolving-congestion prescription on lower extremity arterial restenosis in model dogs with diabetes mellitus.

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