E08C0323 Canine Collagen Type II Alpha 1 ELISA kit
Canine Collagen Type II Alpha 1 ELISA kit is suitable for the detection of samples from Canine species. Collagen Type II Alpha 1 can also be called COL2-A1, COL2A1, COL2 A1, SEDC, Chondrocalcin, COL2α1, COL2-α1, COL2 α1.
Product Information | |
Cat. No. | E08C0323 |
Product Name | Canine Collagen Type II Alpha 1 ELISA kit |
Species | Canine |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
COL2a1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-COL2a1 antibody and an COL2a1-HRP conjugate. The assay sample and buffer are incubated together with COL2a1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL2a1 concentration since COL2a1 from samples and COL2a1-HRP conjugate compete for the anti-COL2a1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL2a1 from the sample, fewer sites are left to bind COL2a1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL2a1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-104% | |
Linearity | Diluent Ratio | Range % |
1:2 | 87-105 | |
1:4 | 84-109 | |
1:8 | 81-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between COL2a1 and analogues was observed. | |
E08C0323 has been referenced in the below publications:
Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog.
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