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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Collagen Type II Alpha 1 ELISA kit (E08C0323)

E08C0323 Canine Collagen Type II Alpha 1 ELISA kit

Canine Collagen Type II Alpha 1 ELISA kit is suitable for the detection of samples from Canine species. Collagen Type II Alpha 1 can also be called COL2-A1, COL2A1, COL2 A1, SEDC, Chondrocalcin, COL2α1, COL2-α1, COL2 α1.

Products

Specifications of Canine Collagen Type II Alpha 1 ELISA kit

Product Information

Cat. No.

E08C0323

Product Name

Canine Collagen Type II Alpha 1 ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

COL2a1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-COL2a1 antibody and an COL2a1-HRP conjugate. The assay sample and buffer are incubated together with COL2a1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL2a1 concentration since COL2a1 from samples and COL2a1-HRP conjugate compete for the anti-COL2a1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL2a1 from the sample, fewer sites are left to bind COL2a1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL2a1 concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Collagen Type II Alpha 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-104%

Linearity

Diluent Ratio

Range %

1:2

87-105

1:4

84-109

1:8

81-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between COL2a1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Canine Collagen Type II Alpha 1 ELISA kit

Summary of the Assay Procedure for Canine Collagen Type II Alpha 1 ELISA kit

Citations of Canine Collagen Type II Alpha 1 ELISA kit

E08C0323 has been referenced in the below publications:

Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog.

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