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E08A0413 Canine Aggrecan ELISA Kit

Canine Aggrecan ELISA kit is suitable for the detection of samples from canine species. Aggrecan can also be called as ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK.

Specifications Of E08A0413 Aggrecan ELISA Kit

Product's Information

Cat. NO.

E08A0413

Product Name

Canine Aggrecan ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AGC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AGC antibody and an AGC-HRP conjugate. The assay sample and buffer are incubated together with AGC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGC concentration since AGC from samples and AGC-HRP conjugate compete for the anti-AGC antibody binding site. Since the number of sites is limited, as more sites are occupied by AGC from the sample, fewer sites are left to bind AGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGC concentration in each sample is interpolated from this standard curve.


Quality Control On Aggrecan ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

90-111

Linearity

Diluent ratio

Range %

1:2

92-109

1:4

87-106

1:8

86-104

Specificity/

Cross-reactivity

No significant cross-reactivity or interference between AGC and analogues was observed.


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Summary Of The Assay Procedures For E08A0413 Aggrecan ELISA Kit

Summary Of The Assay Procedures For E08A0413 Aggrecan ELISA Kit

Citations Of Canine Aggrecan ELISA Kit

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