Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

BlueGene Biotech Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

E08T0047 Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

The Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit can be used to identify samples from the Canine species. Tissue Inhibitor of Metalloproteinases 1 can also be called TIMP 1, Clgi, Collagenase inhibitor, EPA, EPO, Erythroid Potentiating Activity, Fibroblast collagenase inhibitor, HC, Metalloproteinase inhibitor 1, Metalloproteinase inhibitor 1 precursor, TIMP, TIMP metallopeptidase inhibitor 1, TIMP1 protein, Tissue Inhibitor of Metalloproteinase 1, Tissue inhibitor of metalloproteinases, Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor.

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Specifications of Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

Product Information
Cat. No.	E08T0047
Product Name	Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit
Species	Canine
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

TIMP 1 ELISA kit uses an anti-TIMP 1 antibody and an TIMP 1-HRP conjugate in a competitive enzyme immunoassay method. TIMP 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TIMP 1 antibody binding site between TIMP 1 from samples and TIMP 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TIMP 1. Since the number of sites is limited, as more sites are occupied by TIMP 1 from the sample, fewer sites are left to bind TIMP 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIMP 1 concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Tissue Inhibitor of Metalloproteinases 1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between TIMP 1 and analogues was observed.