Canine 26S proteasome ELISA kit is suitable for the detection of samples from Canine species. 26S proteasome can also be called as 26S PSM.
Specifications Of E08A0669 Canine 26S PSM ELISA Kit
Porduct's Information | |
Cat.NO. | E08A0669 |
Porduct's Name | Canine 26S proteasome ELISA kit |
Species | Canine |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
26S PSM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-26S PSM antibody and an 26S PSM-HRP conjugate. The assay sample and buffer are incubated together with 26S PSM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 26S PSM concentration since 26S PSM from samples and 26S PSM-HRP conjugate compete for the anti-26S PSM antibody binding site. Since the number of sites is limited, as more sites are occupied by 26S PSM from the sample, fewer sites are left to bind 26S PSM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 26S PSM concentration in each sample is interpolated from this standard curve. |
Quality Control On Canine 26S PSM ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 88-106 | |
Linearity | Diluent ratio | Range % |
1:2 | 86-105 | |
1:4 | 83-108 | |
1:8 | 84-110 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between 26S PSM and analogues was observed. | |
BlueGene Biotech Product Show
Summary Of The Assay Procedures For E08A0669 Canine 26S PSM ELISA Kit
Citations Of Canine 26S Proteasome ELISA Kit
26S proteasome and insulin-like growth factor-1 in serum of dogs suffering from malignant tumors
Evaluation of serum insulin-like growth factor-1 and 26S proteasome concentrations in healthy dogs and dogs with chronic diseases depending on body condition score
