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BlueGene Biotech Canine Myeloperoxidase ELISA kit (E08M0032)

E08M0032 Canine Myeloperoxidase ELISA kit

The Canine Myeloperoxidase ELISA kit can be used to identify samples from the Canine species. Myeloperoxidase can also be called 89 kDa myeloperoxidase, 84 kDa yeloperoxidase, Myeloperoxidase heavy chain, PERM, MPO.

Products

Specifications of Canine Myeloperoxidase ELISA kit

Product Information

Cat. No.

E08M0032

Product Name

Canine Myeloperoxidase ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MPO ELISA kit uses an anti-MPO antibody and an MPO-HRP conjugate in a competitive enzyme immunoassay method. MPO-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MPO antibody binding site between MPO from samples and MPO-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MPO. Since the number of sites is limited, as more sites are occupied by MPO from the sample, fewer sites are left to bind MPO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MPO concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Myeloperoxidase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MPO and analogues was observed.


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Summary of the Assay Procedure for Canine Myeloperoxidase ELISA kit

Summary of the Assay Procedure for Canine Myeloperoxidase ELISA kit

Citations of Canine Myeloperoxidase ELISA kit

E08M0032 has been referenced in the below publications:

Experimental peri-implant mucositis at different implant surfaces.

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