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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Neurotrophin 3 ELISA kit (E08N0018)

E08N0018 Canine Neurotrophin 3 ELISA kit

The Canine Neurotrophin 3 ELISA kit can be used to identify samples from the Canine species. Neurotrophin 3 can also be called HDNF, MGC129711, Nerve growth factor 2, Neurotrophic factor, Neurotrophin 3 precursor, Neurotrophin-3, Neurotrophin3, NGF 2, NGF-2, NGF2, NT 3, NT-3, NT3, NTF 3, NTF3.

Products

Specifications of Canine Neurotrophin 3 ELISA kit

Product Information

Cat. No.

E08N0018

Product Name

Canine Neurotrophin 3 ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

250-5000pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NT3 ELISA kit uses an anti-NT3 antibody and an NT3-HRP conjugate in a competitive enzyme immunoassay method. NT3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NT3 antibody binding site between NT3 from samples and NT3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NT3. Since the number of sites is limited, as more sites are occupied by NT3 from the sample, fewer sites are left to bind NT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT3 concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Neurotrophin 3 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NT3 and analogues was observed.


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Summary of the Assay Procedure for Canine Neurotrophin 3 ELISA kit

Summary of the Assay Procedure for Canine Neurotrophin 3 ELISA kit

Citations of Canine Neurotrophin 3 ELISA kit

E08N0018 has been referenced in the below publications:

Effects of Electroacupuncture on Plasma Concentrations of Three Related Neurotrophic Factors in Normal Dogs.

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