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E08A0643 Canine Activin A ELISA Kit

Canine Activin A ELISA kit is suitable for the detection of samples from Canine species. Activin A can also be called as ACV-A; Activin Beta A Beta A Homodimer.

Specifications Of E08A0643 Canine ACA A ELISA Kit

Porduct's Information

Cat.NO.

E08A0643

Porduct's Name

Canine Activin A ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1 pg/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ACA A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ACA A antibody and an ACA A-HRP conjugate. The assay sample and buffer are incubated together with ACA A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACA A concentration since ACA A from samples and ACA A-HRP conjugate compete for the anti-ACA A antibody binding site. Since the number of sites is limited, as more sites are occupied by ACA A from the sample, fewer sites are left to bind ACA A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACA A concentration in each sample is interpolated from this standard curve.


Quality Control On Canine ACA A ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

86-115

Linearity

Diluent ratio

Range %

1:2

84-114

1:4

87-117

1:8

80-112

Specificity/

Cross-reactivity

No significant cross-reactivity or interference between ACA A and analogues was observed.


BlueGene Biotech Product Show

Summary Of The Assay Procedures For E08A0643 Canine ACA A ELISA Kit

Summary Of The Assay Procedures For E08A0643 Canine ACA A ELISA Kit

Citations Of Canine Activin A ELISA Kit

Metoprolol inhibits profibrotic remodeling of epicardial adipose tissue in a canine model of chronic obstructive sleep apnea

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