E08A0643 Canine Activin A ELISA kit
Canine Activin A ELISA kit is suitable for the detection of samples from Canine species. Activin A can also be called ACV-A, Activin Beta A Beta A Homodimer, ACVA, Activin-A, ACAA, ACA A, ACA-A, ACV A, ACV-A.
Specifications of Canine Activin A ELISA kit
Product Information | |
Cat. No. | E08A0643 |
Product Name | Canine Activin A ELISA kit |
Species | Canine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ACA A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ACA A. Standards or samples are then added to the microtiter plate wells and ACA A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ACA A present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ACA A are added to each well to “sandwich” the ACA A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACA A and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACA A concentration in each sample is interpolated from this standard curve. |
Quality Control on Canine Activin A ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 86-115% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-114 | |
1:4 | 87-117 | |
1:8 | 80-112 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ACA A and analogues was observed. | |
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Summary of the Assay Procedure for Canine Activin A ELISA kit
Citations of Canine Activin A ELISA kit
E08A0643 has been referenced in the below publications:
Metoprolol inhibits profibrotic remodeling of epicardial adipose tissue in a canine model of chronic obstructive sleep apnea.
