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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Hyaluronan synthase 2 ELISA Kit

E08H1412 Canine Hyaluronan synthase 2 ELISA Kit

The Canine Hyaluronan synthase 2 ELISA Kit can be used to identify samples from the Canine species. Hyaluronan synthase 2 can also be called HA synthase 2, HAS2_HUMAN, Hyaluronan synthase 2, Hyaluronate synthase 2, Hyaluronic acid synthase 2, HAS2, HAS 2, HAS-2.

Specifications of Canine Hyaluronan synthase 2 ELISA Kit

Product Information

Cat. No.

E08H1412

Product Name

Canine Hyaluronan synthase 2 ELISA Kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

25-500ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HAS2 ELISA kit uses an anti-HAS2 antibody and an HAS2-HRP conjugate in a competitive enzyme immunoassay method. HAS2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HAS2 antibody binding site between HAS2 from samples and HAS2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HAS2. Since the number of sites is limited, as more sites are occupied by HAS2 from the sample, fewer sites are left to bind HAS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HAS2 concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Hyaluronan synthase 2 ELISA Kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HAS2 and analogues was observed.


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Summary of the Assay Procedure for Canine Hyaluronan synthase 2 ELISA Kit

Summary of the Assay Procedure for Canine Hyaluronan synthase 2 ELISA Kit

Citations of Canine Hyaluronan synthase 2 ELISA Kit

E08H1412 has been referenced in the below publications:

Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog.

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