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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Matrix Metalloproteinase 3 ELISA kit (E08M0016)

E08M0016 Canine Matrix Metalloproteinase 3 ELISA kit

The Canine Matrix Metalloproteinase 3 ELISA kit is suitable for the detection of samples from Canine species. Matrix Metalloproteinase 3 can also be called SL1, STMY, STMY1, STR1, Progelatinase, Stromelysin 1, Transin-1, MMP3, MMP 3, MMP-3.

Products

Specifications of Canine Matrix Metalloproteinase 3 ELISA kit

Product Information

Cat. No.

E08M0016

Product Name

Canine Matrix Metalloproteinase 3 ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MMP3 ELISA kit uses an anti-MMP3 antibody and an MMP3-HRP conjugate in a competitive enzyme immunoassay method. MMP3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MMP3 antibody binding site between MMP3 from samples and MMP3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MMP3. Since the number of sites is limited, as more sites are occupied by MMP3 from the sample, fewer sites are left to bind MMP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP3 concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Matrix Metalloproteinase 3 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

90-104%

Linearity

Diluent Ratio

Range %

1:2

89-105

1:4

87-106

1:8

84-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MMP3 and analogues was observed.



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Summary of the Assay Procedure for Canine Matrix Metalloproteinase 3 ELISA kit

Summary of the Assay Procedure for Canine Matrix Metalloproteinase 3 ELISA kit

Citations of Canine Matrix Metalloproteinase 3 ELISA kit

E08M0016 has been referenced in the below publications:

Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog.

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