E07C0376 Porcine Cholecystokinin ELISA kit
The Porcine Cholecystokinin ELISA kit is suitable for detecting samples from porcine species. Cholecystokinin can also be called CCK, CCK-PZ.
Specifications of Porcine Cholecystokinin ELISA kit
Product Information | |
Cat. No. | E07C0376 |
Product Name | Porcine Cholecystokinin ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CCK ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CCK antibody and an CCK-HRP conjugate. The assay sample and buffer are incubated together with CCK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCK concentration since CCK from samples and CCK-HRP conjugate compete for the anti-CCK antibody binding site. Since the number of sites is limited, as more sites are occupied by CCK from the sample, fewer sites are left to bind CCK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCK concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Cholecystokinin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 96-113% | |
Linearity | Diluent Ratio | Range % |
1:2 | 97-110 | |
1:4 | 95-108 | |
1:8 | 96-104 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CCK and analogues was observed. | |
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Summary of the Assay Procedure for Porcine Cholecystokinin ELISA kit
Citations of Porcine Cholecystokinin ELISA kit
E07C0376 has been referenced in the below publications:
The heat treatment and the gelation are strong determinants of the kinetics of milk proteins digestion and of the peripheral availability of amino acids.
Acid and rennet gels exhibit strong differences in the kinetics of milk protein digestion and amino acid bioavailability.
