E07I0023 Porcine Interleukin 10 ELISA kit
The Porcine Interleukin 10 ELISA kit can be used to identify samples from the porcine species. Interleukin 10 can also be called IL 10, Interleukin-10 precursor, Cytokine synthesis inhibitory factor, CSIF, IL10A, Interleukin10, TGIF.
Product Information | |
Cat. No. | E07I0023 |
Product Name | Porcine Interleukin 10 ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 10 ELISA kit uses an anti-IL 10 antibody and an IL 10-HRP conjugate in a competitive enzyme immunoassay method. IL 10-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL 10 antibody binding site between IL 10 from samples and IL 10-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 10. Since the number of sites is limited, as more sites are occupied by IL 10 from the sample, fewer sites are left to bind IL 10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 10 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 10 and analogues was observed. | |
E07I0023 has been referenced in the below publications：
Effects of feeding an herbal preparation to sows on immunological performance of offspring.
Effects of direct hemoperfusion with polyvinylidene difluoride-serine column in experimental sepsis.
蜂胶乙醇提取物对断奶仔猪血液生理及免疫功能的影响。
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