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BlueGene Biotech Human Macrophage Inflammatory Protein 1β ELISA kit

E01M0206 Human Macrophage Inflammatory Protein 1β ELISA kit

The Human Macrophage Inflammatory Protein 1β ELISA kit can be used to identify samples from the human species. Macrophage Inflammatory Protein 1β can also be called MIP1β, CCL4L1, MIP-1β, AT744.2, CCL4L, LAG-1, LAG1, SCYA4L, SCYA4L1, MIP-1-beta, SCYA4L2, C-C motif chemokine ligand 4 like 1, Chemokine (C-C motif) ligands 4, CCL4, ACT2, G-26, MIP1-B, SCYA4, Chemokine C-C-Motif Ligand 4, Small Inducible Cytokine A4, Homologous To Mouse Mip-1b.

Specifications of Human Macrophage Inflammatory Protein 1β ELISA kit

Product Information

Cat. No.

E01M0206

Product Name

Human Macrophage Inflammatory Protein 1β ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MIP1β ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MIP1β. Standards or samples are then added to the microtiter plate wells and MIP1β if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MIP1β present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MIP1β are added to each well to “sandwich” the MIP1β immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MIP1β and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP1β concentration in each sample is interpolated from this standard curve.


Quality Control on Human Macrophage Inflammatory Protein 1β ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MIP1β and analogues was observed.


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Summary of the Assay Procedure for Human Macrophage Inflammatory Protein 1β ELISA kit

Summary of the Assay Procedure for Human Macrophage Inflammatory Protein 1β ELISA kit

Citations of Human Macrophage Inflammatory Protein 1β ELISA kit

E01M0206 has been referenced in the below publications:

EXPRESSION AND CORRELATION OF HEME OXYGENASE-1 AND MACROPHAGE INFLAMMATORY PROTEIN-1β IN CORONARY HEART DISEASE.

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