E01C2523 Human citrullinated histone H3 ELISA kit
The Human citrullinated histone H3 ELISA kit can be used to identify samples from the human species. citrullinated histone H3 can also be called H3Cit, H3C1,Histone H3.1.
Specifications of Human citrullinated histone H3 ELISA kit
Product Information | |
Cat. No. | E01C2523 |
Product Name | Human citrullinated histone H3 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
H3Cit ELISA kit uses an anti-H3Cit antibody and an H3Cit-HRP conjugate in a competitive enzyme immunoassay method. H3Cit-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-H3Cit antibody binding site between H3Cit from samples and H3Cit-HRP conjugate, the intensity of the color is inversely proportional to the concentration of H3Cit. Since the number of sites is limited, as more sites are occupied by H3Cit from the sample, fewer sites are left to bind H3Cit-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The H3Cit concentration in each sample is interpolated from this standard curve. |
Quality Control on Human citrullinated histone H3 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between H3Cit and analogues was observed. | |
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Summary of the Assay Procedure for Human citrullinated histone H3 ELISA kit
Citations of Human citrullinated histone H3 ELISA kit
E01C2523 has been referenced in the below publications：
NETosis is associated with the severity of aortic stenosis: Links with inflammation.
Quantification of citrullinated histones: Development of an improved assay to reliably quantify nucleosomal H3Cit in human plasma.
