En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human citrullinated histone H3 ELISA kit (E01C2523)

  • Epigenetics and Nuclear Signaling

E01C2523 Human citrullinated histone H3 ELISA kit

The Human citrullinated histone H3 ELISA kit can be used to identify samples from the human species. citrullinated histone H3 can also be called H3Cit, H3C1,Histone H3.1.

Products

Specifications of Human citrullinated histone H3 ELISA kit

Product Information

Cat. No.

E01C2523

Product Name

Human citrullinated histone H3 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

H3Cit ELISA kit uses an anti-H3Cit antibody and an H3Cit-HRP conjugate in a competitive enzyme immunoassay method. H3Cit-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-H3Cit antibody binding site between H3Cit from samples and H3Cit-HRP conjugate, the intensity of the color is inversely proportional to the concentration of H3Cit. Since the number of sites is limited, as more sites are occupied by H3Cit from the sample, fewer sites are left to bind H3Cit-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The H3Cit concentration in each sample is interpolated from this standard curve. Similarly, the Human Hepcidin ELISA Kit uses a competitive enzyme immunoassay method with an anti-hepcidin antibody and a hepcidin-HRP conjugate to measure hepcidin levels in human samples, with the color intensity inversely proportional to the hepcidin concentration.



Quality Control on Human citrullinated histone H3 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between H3Cit and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human citrullinated histone H3 ELISA kit

Summary of the Assay Procedure for Human citrullinated histone H3 ELISA kit

Citations of Human citrullinated histone H3 ELISA kit

E01C2523 has been referenced in the below publications:

NETosis is associated with the severity of aortic stenosis: Links with inflammation.

Quantification of citrullinated histones: Development of an improved assay to reliably quantify nucleosomal H3Cit in human plasma.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.