E01A0032 Human Artemin ELISA kit
Human Artemin ELISA kit is suitable for the detection of samples from human species. Artemin can also be called ARTN, ENOVIN, EVN, NBN, artemin, ART.
Specifications of Human Artemin ELISA kit
Product Information | |
Cat. No. | E01A0032 |
Product Name | Human Artemin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ART ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ART antibody and an ART-HRP conjugate. The assay sample and buffer are incubated together with ART-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ART concentration since ART from samples and ART-HRP conjugate compete for the anti-ART antibody binding site. Since the number of sites is limited, as more sites are occupied by ART from the sample, fewer sites are left to bind ART-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ART concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Artemin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 95-104 | |
1:4 | 93-108 | |
1:8 | 94-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ART and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Artemin ELISA kit
Citations of Human Artemin ELISA kit
E01A0015 has been referenced in the below publications:
Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression.
Acute exercise prevents the development of neuropathic pain and the sprouting of non-peptidergic (GDNF- and arteminresponsive) c-fibers after spinal cord injury.
