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BlueGene Biotech Human 6-kDa Early Secretory Antigenic Target ELISA Kit

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E01E0516 Human 6-kDa Early Secretory Antigenic Target ELISA Kit

The Human 6-kDa Early Secretory Antigenic Target ELISA Kit can be used to identify samples from the human species. 6-kDa Early Secretory Antigenic Target can also be called Early Secreted Antigenic Target 6 kDa.

Specifications of Human 6-kDa Early Secretory Antigenic Target ELISA Kit

Product Information

Cat. No.

E01E0516

Product Name

Human 6-kDa Early Secretory Antigenic Target ELISA Kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ESAT-6 ELISA kit uses an anti-ESAT-6 antibody and an ESAT-6-HRP conjugate in a competitive enzyme immunoassay method. ESAT-6-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ESAT-6 antibody binding site between ESAT-6 from samples and ESAT-6-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ESAT-6. Since the number of sites is limited, as more sites are occupied by ESAT-6 from the sample, fewer sites are left to bind ESAT-6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ESAT-6 concentration in each sample is interpolated from this standard curve.


Quality Control on Human 6-kDa Early Secretory Antigenic Target ELISA Kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ESAT-6 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human 6-kDa Early Secretory Antigenic Target ELISA Kit

Summary of the Assay Procedure for Human 6-kDa Early Secretory Antigenic Target ELISA Kit

Citations of Human 6-kDa Early Secretory Antigenic Target ELISA Kit

E01E0516 has been referenced in the below publications:

Values of Early Secretory Antigenic Target 6 and Lipoarabinomannan in Diagnosis of Tuberculous Meningitis.

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