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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Total Endothelin ELISA kit (E02E0182)

E02E0182 Rat Total Endothelin ELISA kit

The Rat Total Endothelin ELISA kit can be used to identify samples from the rat species. Total Endothelin can also be called ET.

Products

Specifications of Rat Total Endothelin ELISA kit

Product Information

Cat. No.

E02E0182

Product Name

Rat Total Endothelin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ET ELISA kit uses an anti-ET antibody and an ET-HRP conjugate in a competitive enzyme immunoassay method. ET-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ET antibody binding site between ET from samples and ET-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ET. Since the number of sites is limited, as more sites are occupied by ET from the sample, fewer sites are left to bind ET-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ET concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Total Endothelin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ET and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Total Endothelin ELISA kit

Summary of the Assay Procedure for Rat Total Endothelin ELISA kit

Citations of Rat Total Endothelin ELISA kit

E02E0182 has been referenced in the below publications:

Role of plateau hypoxia hypothermia complex damage to the vascular endothelial cells and the protective effect.

穴位埋线对高血压大鼠血压、血浆内皮素及血清-氧化氮的影响。

Influence of Jianpi Yiqi Huoxue Decoction on Vasomotor Factors Expression in Vascular Endothelium of Spontaneously Hypertensive Rats.

Effects of rapid entrance into plateau area on the vascular endothelial function and the relationship to acute mountain sickness.

Effects of bosentan on endothelin-l of pulmonary fibrosis rats.

Influence of Endothelial Function and Blood Rheology in Diabetic Rats by Paeonol and Tanshinol.


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