E02E0182 Rat Total Endothelin ELISA kit
The Rat Total Endothelin ELISA kit can be used to identify samples from the rat species. Total Endothelin can also be called ET.
Specifications of Rat Total Endothelin ELISA kit
Product Information | |
Cat. No. | E02E0182 |
Product Name | Rat Total Endothelin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ET ELISA kit uses an anti-ET antibody and an ET-HRP conjugate in a competitive enzyme immunoassay method. ET-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ET antibody binding site between ET from samples and ET-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ET. Since the number of sites is limited, as more sites are occupied by ET from the sample, fewer sites are left to bind ET-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ET concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Total Endothelin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ET and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Total Endothelin ELISA kit
Citations of Rat Total Endothelin ELISA kit
E02E0182 has been referenced in the below publications：
Role of plateau hypoxia hypothermia complex damage to the vascular endothelial cells and the protective effect.
穴位埋线对高血压大鼠血压、血浆内皮素及血清-氧化氮的影响。
Influence of Jianpi Yiqi Huoxue Decoction on Vasomotor Factors Expression in Vascular Endothelium of Spontaneously Hypertensive Rats.
Effects of rapid entrance into plateau area on the vascular endothelial function and the relationship to acute mountain sickness.
Effects of bosentan on endothelin-l of pulmonary fibrosis rats.
Influence of Endothelial Function and Blood Ｒheology in Diabetic Ｒats by Paeonol and Tanshinol.
