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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Transforming Growth Factor β1 ELISA kit (E02T0009)

E02T0009 Rat Transforming Growth Factor β1 ELISA kit

The Rat Transforming Growth Factor β1 ELISA kit can be used to identify samples from the rat species. Transforming Growth Factor β1 can also be called CED, DPD1, TGF beta 1, TGF beta, TGF beta 1 protein, TGF-beta 1 protein, TGF-beta-1, TGF-beta-5, TGF-beta1, TGFB, Tgfb-1, tgfb1, TGFbeta, TGFbeta1, Transforming Growth Factor b1, Transforming Growth Factor beta 1, Transforming growth factor beta 1a, transforming growth factor beta-1, transforming growth factor, beta 1.

Products

Specifications of Rat Transforming Growth Factor β1 ELISA kit

Product Information

Cat. No.

E02T0009

Product Name

Rat Transforming Growth Factor β1 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TGFβ1 ELISA kit uses an anti-TGFβ1 antibody and an TGFβ1-HRP conjugate in a competitive enzyme immunoassay method. TGFβ1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TGFβ1 antibody binding site between TGFβ1 from samples and TGFβ1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TGFβ1. Since the number of sites is limited, as more sites are occupied by TGFβ1 from the sample, fewer sites are left to bind TGFβ1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFβ1 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Transforming Growth Factor β1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TGFβ1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Transforming Growth Factor β1 ELISA kit

Summary of the Assay Procedure for Rat Transforming Growth Factor β1 ELISA kit

Citations of Rat Transforming Growth Factor β1 ELISA kit

E02T0009 has been referenced in the below publications:

Nonalcoholic fatty liver tissue of hepatitis TNFΑ, TGFΒ1, LEPTIN and MAPK expression and drug intervention.

THE STUDY OF PREVENTIVE AND THERAPEUTIC EFFECTS OF TRIMETAZIDINE HYDROCHLORIDE ON RAT RENAL INTERSTITIAL FIBROSIS.

Identification of Chronic Atrophic Gastritis Model Rats Differentially Expressed Proteins in Gastric Mucosa by Quantitative Proteomics and Research for Effect Targets of Chinese Medicine.

Discussion of changes of TGF-β 1,Smad-4,α-SMA and PDGF in DN rat liver before and after treatment in traditional Chinese Medicine based on the“liver and kidney share the same origin”.

Study of the Effects of Berberine Hydrochloride on Vascular Smooth Muscle Cells in the Type 2 Diabetes Mellitus.

The renal damage caused by advanced glycation end products relating to activation of Na+ /H +exchanger 1.


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