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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Growth Hormone ELISA kit

E14G0165 Sheep Growth Hormone ELISA kit

Sheep Growth Hormone ELISA kit is suitable for the detection of samples from sheep species. Growth Hormone can also be called GH1, GH-N, GHN, hGH-N, Somatotropin, Hygetropin, Jintropin, Kigtropin, Pituitary Growth Hormone, Growth Hormone, Normal.

Specifications of Sheep Growth Hormone ELISA kit

Product Information

Cat. No.

E14G0165

Product Name

Sheep Growth Hormone ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GH. Standards or samples are then added to the microtiter plate wells and GH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GH are added to each well to “sandwich” the GH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GH concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Growth Hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GH and analogues was observed.


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Summary of the Assay Procedure for Sheep Growth Hormone ELISA kit

Summary of the Assay Procedure for Sheep Growth Hormone ELISA kit

Citations of Sheep Growth Hormone ELISA kit

E14G0165 has been referenced in the below publications:

The Concentration Variation Regularity of GH and IGF-1 in the Peripheral blood of Suffolk Sheeps.

The effect of diets with different protein contents on the IGF 1, GH and growth in sheep.

L-Arginine Modulates Maternal Hormonal Profiles and Neonatal Traits during Two Stages of Pregnancy in Sheep.

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