Sheep Insulin ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Sheep Insulin ELISA kit

E14I0004 Sheep Insulin ELISA kit

Sheep Insulin ELISA kit is suitable for the detection of samples from sheep species. Insulin can also be called ILPR, Insulin A chain, Insulin B chain, IRDN, INS.

Products

Specifications of Sheep Insulin ELISA kit

Product Information
Cat. No.	E14I0004
Product Name	Sheep Insulin ELISA kit
Species	Sheep
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

INS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-INS antibody and an INS-HRP conjugate. The assay sample and buffer are incubated together with INS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INS concentration since INS from samples and INS-HRP conjugate compete for the anti-INS antibody binding site. Since the number of sites is limited, as more sites are occupied by INS from the sample, fewer sites are left to bind INS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INS concentration in each sample is interpolated from this standard curve.

Quality Control on Sheep Insulin ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	87-105%
Linearity	Diluent Ratio	Range %
	1:2	84-108
	1:4	81-107
	1:8	80-110
Specificity/Cross-reactivity	No significant cross-reactivity or interference between INS and analogues was observed.