E14N0063 Sheep Non ester Fatty Acid ELISA kit
Sheep Non ester Fatty Acid ELISA kit is suitable for the detection of samples from sheep species. Non ester Fatty Acid can also be called FFA, free fatty acid, NEFA.
Specifications of Sheep Non ester Fatty Acid ELISA kit
Product Information | |
Cat. No. | E14N0063 |
Product Name | Sheep Non ester Fatty Acid ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 µmol/L |
Sensitivity | 0.1 µmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 µmol/L | 1 vial |
STANDARD B (0.5mL) | 0.5 µmol/L | 1 vial |
STANDARD C (0.5mL) | 1.0 µmol/L | 1 vial |
STANDARD D (0.5mL) | 2.5 µmol/L | 1 vial |
STANDARD E (0.5mL) | 5 µmol/L | 1 vial |
STANDARD F (0.5mL) | 10 µmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NEFA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-NEFA antibody and an NEFA-HRP conjugate. The assay sample and buffer are incubated together with NEFA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NEFA concentration since NEFA from samples and NEFA-HRP conjugate compete for the anti-NEFA antibody binding site. Since the number of sites is limited, as more sites are occupied by NEFA from the sample, fewer sites are left to bind NEFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NEFA concentration in each sample is interpolated from this standard curve. |
Quality Control on Sheep Non ester Fatty Acid ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NEFA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Sheep Non ester Fatty Acid ELISA kit
Citations of Sheep Non ester Fatty Acid ELISA kit
E14N0063 has been referenced in the below publications:
Effects of Eucommia ulmoides Leaves on Sheep Lipid Metabolism and the Mechanism.
