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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Glucagon ELISA kit

E14G0101 Sheep Glucagon ELISA kit

Sheep Glucagon ELISA kit is suitable for the detection of samples from sheep species. Glucagon can also be called GC, GCG, GLP1, GLP2, GRPP, glucagon.

Specifications of Sheep Glucagon ELISA kit

Product Information

Cat. No.

E14G0101

Product Name

Sheep Glucagon ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GC antibody and an GC-HRP conjugate. The assay sample and buffer are incubated together with GC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GC concentration since GC from samples and GC-HRP conjugate compete for the anti-GC antibody binding site. Since the number of sites is limited, as more sites are occupied by GC from the sample, fewer sites are left to bind GC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GC concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Glucagon ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Glucagon ELISA kit

Summary of the Assay Procedure for Sheep Glucagon ELISA kit

Citations of Sheep Glucagon ELISA kit

E14G0101 has been referenced in the below publications:

Effects of Eucommia ulmoides Leaves on Glucose Metabolism and Related Gene Expression in Sheep Liver.

Effects of α-Lipoic Acid on Growth Performance,Antioxidant and Immunity of Sheep under Heat Stress.

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