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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Insulin like Growth Factor 1 ELISA kit (E14I0009)

E14I0009 Sheep Insulin like Growth Factor 1 ELISA kit

Sheep Insulin like Growth Factor 1 ELISA kit is suitable for the detection of samples from sheep species. Insulin like Growth Factor 1 can also be called IBP1, IGF IA, IGF IB, IGFI, IGFIA, Insulin Like Growth Factor 1, Insulin like growth factor IA, Insulin like growth factor IB, Mechano growth factor, MGF, Mechano growth factor, MGF, Somatomedia C, Somatomedin C.

Products

Specifications of Sheep Insulin like Growth Factor 1 ELISA kit

Product Information

Cat. No.

E14I0009

Product Name

Sheep Insulin like Growth Factor 1 ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IGF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IGF1 antibody and an IGF1-HRP conjugate. The assay sample and buffer are incubated together with IGF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF1 concentration since IGF1 from samples and IGF1-HRP conjugate compete for the anti-IGF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF1 from the sample, fewer sites are left to bind IGF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF1 concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Insulin like Growth Factor 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IGF1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Insulin like Growth Factor 1 ELISA kit

Summary of the Assay Procedure for Sheep Insulin like Growth Factor 1 ELISA kit

Citations of Sheep Insulin like Growth Factor 1 ELISA kit

E14I0009 has been referenced in the below publications:

The Concentration Variation Regularity of GH and IGF-1 in the Peripheral blood of Suffolk Sheeps.

The effect of diets with different protein contents on the IGF 1, GH and growth in sheep.

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