En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Inositol Triphosphate ELISA kit

E14I0011 Sheep Inositol Triphosphate ELISA kit

Sheep Inositol Triphosphate ELISA kit is suitable for the detection of samples from sheep species. Inositol Triphosphate can also be called InsP3, Inositol 1,4,5-Trisphosphate, Triphosphoinositol, IP3.

Specifications of Sheep Inositol Triphosphate ELISA kit

Product Information

Cat. No.

E14I0011

Product Name

Sheep Inositol Triphosphate ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IP3 antibody and an IP3-HRP conjugate. The assay sample and buffer are incubated together with IP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IP3 concentration since IP3 from samples and IP3-HRP conjugate compete for the anti-IP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by IP3 from the sample, fewer sites are left to bind IP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IP3 concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Inositol Triphosphate ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IP3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Inositol Triphosphate ELISA kit

Summary of the Assay Procedure for Sheep Inositol Triphosphate ELISA kit

Citations of Sheep Inositol Triphosphate ELISA kit

E14I0011 has been referenced in the below publications:

Calcium Sensing Receptor Expression in Ovine Amniotic Fluid Mesenchymal Stem Cells and the Potential Role of R-568 during Osteogenic Differentiation.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.