E14M0240 Sheep Mannma binding protein/mannan binding lectin ELISA kit
Sheep Mannma binding protein/mannan binding lectin ELISA kit is suitable for the detection of samples from sheep species. Mannma binding protein/mannan binding lectin can also be called MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, MBL.
Product Information | |
Cat. No. | E14M0240 |
Product Name | Sheep Mannma binding protein/mannan binding lectin ELISA kit |
Species | Sheep |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MBP/MBL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MBP/MBL antibody and an MBP/MBL-HRP conjugate. The assay sample and buffer are incubated together with MBP/MBL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MBP/MBL concentration since MBP/MBL from samples and MBP/MBL-HRP conjugate compete for the anti-MBP/MBL antibody binding site. Since the number of sites is limited, as more sites are occupied by MBP/MBL from the sample, fewer sites are left to bind MBP/MBL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBP/MBL concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-108 | |
1:4 | 81-107 | |
1:8 | 80-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MBP/MBL and analogues was observed. | |
E14M0240 has been referenced in the below publications:
Construction of Sheep MBL Eukaryotic Expression Vector and Screening of disease resistance associated miRNA.
Study on the relationship between sheep Mannose—binding lectin gene promoter methylation and sheep Mycoplasma pneumoniae infection.
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