E01F0035 Human Fatty Acid Binding Protein 4, Adipocyte ELISA kit
The Human Fatty Acid Binding Protein 4, Adipocyte ELISA kit can be used to identify samples from the human species. Fatty Acid Binding Protein 4, Adipocyte can also be called FABP4, A-FABP, AFABP, ALBP, HEL-S-104, aP2, fatty acid binding protein 4, adipocyte Protein 2.
Product Information | |
Cat. No. | E01F0035 |
Product Name | Human Fatty Acid Binding Protein 4, Adipocyte ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FABP4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FABP4. Standards or samples are then added to the microtiter plate wells and FABP4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FABP4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FABP4 are added to each well to “sandwich” the FABP4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound coFABP4nents. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FABP4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP4 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FABP4 and analogues was observed. | |
E01F0035 has been referenced in the below publications：
The clinical study of metabolic disorders and the correlation with Adipocytokines in PCOS women.
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