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BlueGene Biotech Human Alkaline Phosphatase ELISA kit (E01A0031)

E01A0031 Human Alkaline Phosphatase ELISA kit

Human Alkaline Phosphatase ELISA kit is suitable for the detection of samples from human species. Alkaline Phosphatase can also be called AKP, ALKP, Basic Phosphatase, ALP.

Products

Specifications of Human Alkaline Phosphatase ELISA kit

Product Information

Cat. No.

E01A00031

Product Name

Human Alkaline Phosphatase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ALP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ALP antibody and an ALP-HRP conjugate. The assay sample and buffer are incubated together with ALP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALP concentration since ALP from samples and ALP-HRP conjugate compete for the anti-ALP antibody binding site. Since the number of sites is limited, as more sites are occupied by ALP from the sample, fewer sites are left to bind ALP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALP concentration in each sample is interpolated from this standard curve.


Quality Control on Human Alkaline Phosphatase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-107%

Linearity

Diluent Ratio

Range %

1:2

91-106

1:4

90-103

1:8

89-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ALP and analogues was observed.



BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Alkaline Phosphatase ELISA kit

Summary of the Assay Procedure for Human Alkaline Phosphatase ELISA kit

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E01A0031 Human Alkaline Phosphatase ELISA kit

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