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BlueGene Biotech Human Ovalbumin specific Immunoglobulin E ELISA kit (E01O0012)

E01O0012 Human Ovalbumin specific Immunoglobulin E ELISA kit

The Human Ovalbumin specific Immunoglobulin E ELISA kit can be used to identify samples from the human species. Ovalbumin specific Immunoglobulin E can also be called OVA-slgE.

Products

Specifications of Human Ovalbumin specific Immunoglobulin E ELISA kit

Product Information

Cat. No.

E01O0012

Product Name

Human Ovalbumin specific Immunoglobulin E ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

OVA slgE ELISA kit uses an anti-OVA slgE antibody and an OVA slgE-HRP conjugate in a competitive enzyme immunoassay method. OVA slgE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OVA slgE antibody binding site between OVA slgE from samples and OVA slgE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OVA slgE. Since the number of sites is limited, as more sites are occupied by OVA slgE from the sample, fewer sites are left to bind OVA slgE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OVA slgE concentration in each sample is interpolated from this standard curve.


Quality Control on Human Ovalbumin specific Immunoglobulin E ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between OVA slgE and analogues was observed.


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Summary of the Assay Procedure for Human Ovalbumin specific Immunoglobulin E ELISA kit

Summary of the Assay Procedure for Human Ovalbumin specific Immunoglobulin E ELISA kit

Citations of Human Ovalbumin specific Immunoglobulin E ELISA kit

E01O0012 has been referenced in the below publications:

Lentiviral shRNA against KCa3.1 inhibits allergic response in allergic rhinitis and suppresses mast cell activity via PI3K/AKT signaling pathway.

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