Human Gonadotropin Releasing Hormone ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Gonadotropin Releasing Hormone ELISA kit

E01G0213 Human Gonadotropin Releasing Hormone ELISA kit

The Human Gonadotropin Releasing Hormone ELISA kit can be used to identify samples from the human species. Gonadotropin Releasing Hormone can also be called GNRH1, GNRH, GRH, HH12, LHRH, LNRH, gonadotropin releasing hormone 1, Gonadotropin-Releasing Hormone, Progonadoliberin-1, Progonadoliberin I, GnRH-I, LH-RH I, Gonadoliberin I, Gonadorelin, Gonadotropin-releasing hormone I, Luliberin I, Luteinizing hormone-releasing hormone I, GnRH-associated peptide 1, GnRH-associated peptide I.

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Specifications of Human Gonadotropin Releasing Hormone ELISA kit

Product Information
Cat. No.	E01G0213
Product Name	Human Gonadotropin Releasing Hormone ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

GnRH ELISA kit uses an anti-GnRH antibody and an GnRH-HRP conjugate in a competitive enzyme immunoassay method. GnRH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GnRH antibody binding site between GnRH from samples and GnRH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GnRH. Since the number of sites is limited, as more sites are occupied by GnRH from the sample, fewer sites are left to bind GnRH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GnRH concentration in each sample is interpolated from this standard curve.

Quality Control on Human Gonadotropin Releasing Hormone ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between GnRH and analogues was observed.

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Summary of the Assay Procedure for Human Gonadotropin Releasing Hormone ELISA kit

Citations of Human Gonadotropin Releasing Hormone ELISA kit

E01G0213 has been referenced in the below publications：

Peripheral Inhibitor of AChE, Neostigmine, Prevents the Inflammatory Dependent Suppression of GnRH/LH Secretion during the Follicular Phase of the Estrous Cycle.