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E01A0047 Human Adrenomedullin ELISA Kit

Human Adrenomedullin ELISA kit is suitable for the detection of samples from human species. Adrenomedullin can also be called as AM; Adrenomedullin; ADM; ADM precursor; ADML.

Specifications of E01A0047 Human AM ELISA Kit

Porduct's Information

Cat.NO.

E01A0047

Porduct's Name

Human Adrenomedullin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

100-2500pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AM antibody and an AM-HRP conjugate. The assay sample and buffer are incubated together with AM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AM concentration since AM from samples and AM-HRP conjugate compete for the anti-AM antibody binding site. Since the number of sites is limited, as more sites are occupied by AM from the sample, fewer sites are left to bind AM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AM concentration in each sample is interpolated from this standard curve.


Quality Control On Human AM ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

89-107

Linearity

Diluent ratio

Range %

1:2

95-102

1:4

90-108

1:8

91-112

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AM and analogues was observed.


BlueGene Biotech Product Show

Summary of The Assay Procedures For E01A0047 Human AM ELISA Kit

Summary of The Assay Procedures For E01A0047 Human AM ELISA Kit

Citations of Human Adrenomedullin ELISA Kit

Analysis of the Secretome of Apoptotic Peripheral Blood Mononuclear Cells: Impact of Released Proteins and Exosomes for Tissue Regeneration

Product: E01C0360 Human Complement Component 3 ELISA kit;


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