E01P0041 Human P53 ELISA kit. This kit is for research use only.
Specifications of Human P53 ELISA kit
Product Information | |
Cat. No. | NE01P0041 |
Product Name | Human P53 ELISA kit |
Species | Human |
Product Size | 96 Tests |
Concentration | 78.125-5000pg/mL |
Sensitivity | LOD: 2.4pg/mL LOQ: 19.53pg/mL |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 3 hours and 45 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
Standard (Stock Solution) -1μg/mL | 20 μL | 1 vial |
HRP-conjugated Detection antibody (100×) | 150 μL | 1 vial |
TMB Substrates | 10 mL | 1 vial (Avoid Light) |
Stop Solution | 10 mL | 1 vial |
Wash Solution (100×) | 10 mL | 1 vial |
Diluent Buffer (10×) | 10 mL | 1 vial |
Plate Sealer | 50 ng/mL | 4 pieces |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit adopts the double antibody sandwich Enzyme Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample. |
Quality Control on Human P53 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
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Summary of the Assay Procedure for Human P53 ELISA kit
Citations of Human P53 ELISA kit
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