Name: Human Immunoglobulin G (IgG) ELISA Kit (NE01I0431)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Human Immunoglobulin G (IgG) ELISA Kit (NE01I0431)

NE01I0431 Human Immunoglobulin G (IgG) ELISA Kit

This Human IgG ELISA kit is for research use only, it should not be used in clinical diagnostic procedures. This Kit for the quantitative Measurement of human IgG Residues in Protein Purification Process and End-Product (purified fermentation broth, cell culture supernatant, etc.)

Products

Specifications of Human Immunoglobulin G (IgG) ELISA kit

Product Information
Cat. NO.	NE01I0431
Products Name	Human Immunoglobulin G (IgG) ELISA kit
Species	Human
Product Size	96 Tests
Concentration	156.25-10000 pg/ml
Sensitivity	10.2 pg/ml
Principal	Sandwich ELISA
Sample Volume	100 ul
Sample Type	ELISA Kit for the quantitative Measurement of human IgG Residues in Protein Purification Process, and End-Product (purified fermentation broth, cell culture supernatant, etc.)
Assay Time	3 hours and 15 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
Reagents	Specification	Quantity
Pre-Coated Microplate (Detachable)	96 wells	1 plate (Keep Sealed)
Standard (Stock Solution) -5ug/ml	20ul	1 vial
Detection antibody (100×)	150ul	1 vial
HRP-conjugated antibody(200×)	100ul	1 vial
Diluent Buffer (10×)	10ml	1 vial
TMB Substrates	10ml	1 vial (Avoid Light)
Stop Solution	10ml	1 vial
Wash Solution (100×)	10ml	1 vial
Plate Sealer		4 pieces
Instruction Manual		1

Principle of the Assay

This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of human IgG in samples. The microtiter plate has been pre-coated with goat anti-human antibody , standards or samples are then added to the microtiter plate wells and IgG if present, will bind to the antibody pre-coated wells under specific conditions. After washing, then a coupled biotin-conjugated detection goat anti-human antibody is added to the wells, under 37℃ incubation, to form a precoated antibody-human IgG-detection antibody sandwich complex. After washing, coupled avidin conjugated Horseradish Peroxidase (HRP) is added to the wells, resulting in a cascade amplification effect. Then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total IgG bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of human IgG in samples is then determined by comparing the O.D. of the samples to the standard curve

Quality Control on Human Immunoglobulin G (IgG) ELISA kit

Coefficient of Variance	Intra Variation: 2.7-3.14%
	Inter Variation: 8.4-9.6%
Recovery	88.77-113.22%
Linearity	Diluent Ratio	Range %	Average Linearity %
	1	97.43-98.31	97.87
	1:2	103.01-103.30	103.15
	1:4	97.23-102.06	99.65
	1:8	89.02-101.74	95.38
	1:16	99.86-114.11	106.98
	1:32	101.25-105.87	103.56
	1:64	80.07-96.14	88.11
Specificity/ Cross-reactivity	Sample	Cross-Reactivity（%）
	Mouse IgG	0.349
	Bovine IgG	---
	VERO HCP	---
	293 HCP	---
	MDCK HCP	---
	OVA	---
	chicken anti-SPA	---
	TRF	---