BlueGene E02A0039 Rat Total β Amyloid Protein ELISA Kit

BlueGene Biotech Rat Total β Amyloid Protein ELISA kit

E02A0039 Rat Total β Amyloid Protein ELISA kit

Rat Total β Amyloid Protein ELISA kit is suitable for the detection of samples from Rat species. Total β Amyloid Protein can also be called Aβ, Aβ protein, Amyloid beta-peptide, Beta-amyloid peptide, Beta-amyloid protein, beta Amyloid Protein.

Products

Specifications of Rat Total β Amyloid Protein ELISA kit

Product Information
Cat. No.	E02A0039
Product Name	Rat Total β Amyloid Protein ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/ml
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

AβELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Aβantibody and an A-HRP conjugate. The assay sample and buffer are incubated together with Aβ-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Aβconcentration since Aβfrom samples and Aβ-HRP conjugate compete for the anti-Aβantibody binding site. Since the number of sites is limited, as more sites are occupied by Aβ from the sample, fewer sites are left to bind Aβ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβconcentration in each sample is interpolated from this standard curve.

Quality Control on Rat Total β Amyloid Protein ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	93-106%
Linearity	Diluent Ratio	Range %
	1:2	94-104
	1:4	92-110
	1:8	90-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between Aβ and analogues was observed.