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  • bovine serum albumin elisa 1

BlueGene Biotech Human Low Density Lipoprotein ELISA kit

E01L0020 Human Low Density Lipoprotein ELISA kit

The Human Low Density Lipoprotein ELISA kit can be used to identify samples from the human species. Low Density Lipoprotein can also be called LDL, apolipoprotein, low density, low density lipoprotein, Low density lipoprotein.

Specifications of Human Low Density Lipoprotein ELISA kit

Product Information

Cat. No.

E01L0020

Product Name

Human Low Density Lipoprotein ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/ml

Sensitivity

1.0 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LDL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LDL. Standards or samples are then added to the microtiter plate wells and LDL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LDL present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LDL are added to each well to “sandwich” the LDL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LDL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LDL concentration in each sample is interpolated from this standard curve.


Quality Control on Human Low Density Lipoprotein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LDL and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Low Density Lipoprotein ELISA kit

Summary of the Assay Procedure for Human Low Density Lipoprotein ELISA kit

Citations of Human Low Density Lipoprotein ELISA kit

E01L0020 has been referenced in the below publications:

Effects of Noise on The Hearing And Cardiovascular System In Noise-exposed Workers.

Study on the relationship between homocysteine levels and coronary heart disease in femal.

Effect of different hemodialysis modalities on resistin and nutritional status.

Antioxidant Blueberry Anthocyanins Induce Vasodilation via PI3K/Akt Signaling Pathway in High-Glucose-Induced Human Umbilical Vein Endothelial Cells.

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