E01T0565 Human Thyroid transcription factor 1 ELISA kit
The Human Thyroid transcription factor 1 ELISA kit can be used to identify samples from the human species. Thyroid transcription factor 1 can also be called TTF 1, NKX2-1, Nkx2-1, AV026640, Nkx2.1, T/EBP, Titf1, Ttf-1, BCH, BHC, NK-2, NKX2A, TEBP, TTF1, NMTC1, NK2 homeobox 1.
Product Information | |
Cat. No. | E01T0565 |
Product Name | Human Thyroid transcription factor 1 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TTF 1 ELISA kit uses an anti-TTF 1 antibody and an TTF 1-HRP conjugate in a competitive enzyme immunoassay method. TTF 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TTF 1 antibody binding site between TTF 1 from samples and TTF 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TTF 1. Since the number of sites is limited, as more sites are occupied by TTF 1 from the sample, fewer sites are left to bind TTF 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TTF 1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TTF 1 and analogues was observed. | |
E01T0565 has been referenced in the below publications:
The Research of NKX2-1 with the Biological Effect and Anti-cancer Drugs Resistance in Lung Cancer CeⅡs.
Expression and clinical significance of TTF-1 in the serum of lung adenocarcinoma patients.
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