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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Estrogens ELISA kit

E11E0314 Bovine Estrogens ELISA kit

The Bovine Estrogens ELISA kit can be used to identify samples from the bovine species. Estrogens can also be called ES, oestrogen.

Specifications of Bovine Estrogens ELISA kit

Product Information

Cat. No.

E11E0314

Product Name

Bovine Estrogens ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ES ELISA kit uses an anti-ES antibody and an ES-HRP conjugate in a competitive enzyme immunoassay method. ES-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ES antibody binding site between ES from samples and ES-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ES. Since the number of sites is limited, as more sites are occupied by ES from the sample, fewer sites are left to bind ES-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ES concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Estrogens ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ES and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Estrogens ELISA kit

Summary of the Assay Procedure for Bovine Estrogens ELISA kit

Citations of Bovine Estrogens ELISA kit

E11E0314 has been referenced in the below publications:

奶牛排卵延迟的综合诊断。

Correlation Analysis of IL and Hormone in Serum During Treatment of Persistent Courpus Luteum with Fertility-promoting Power in Dairy Cows.

Rule Analysis of LH,E2 and P4 of Ovulation-Delayed Dairy Cows at Different Stages after Estrus.

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