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  • bovine serum albumin elisa 1

BlueGene Biotech Human Carbohydrate Antigen 125 ELISA kit

E01C0473 Human Carbohydrate Antigen 125 ELISA kit

The Human Carbohydrate Antigen 125 ELISA kit can be used to identify samples from the human species. Carbohydrate Antigen 125 can also be called  MUC16, CA125, mucin 16, cell surface associated.

Specifications of Human Carbohydrate Antigen 125 ELISA kit

Product Information

Cat. No.

E01C0473

Product Name

Human Carbohydrate Antigen 125 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 U/mL

Sensitivity

1.0 U/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 U/mL

1 vial

STANDARD B (0.5mL)

5.0 U/mL

1 vial

STANDARD C (0.5mL)

10 U/mL

1 vial

STANDARD D (0.5mL)

25 U/mL

1 vial

STANDARD E (0.5mL)

50 U/mL

1 vial

STANDARD F (0.5mL)

100 U/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CA125 ELISA kit uses an anti-CA125 antibody and an CA125-HRP conjugate in a competitive enzyme immunoassay method. CA125-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CA125 antibody binding site between CA125 from samples and CA125-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CA125. Since the number of sites is limited, as more sites are occupied by CA125 from the sample, fewer sites are left to bind CA125-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA125 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Carbohydrate Antigen 125 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CA125 and analogues was observed.


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Summary of the Assay Procedure for Human Carbohydrate Antigen 125 ELISA kit

Summary of the Assay Procedure for Human Carbohydrate Antigen 125 ELISA kit

Citations of Human Carbohydrate Antigen 125 ELISA kit

E01C0473 has been referenced in the below publications:

The application of TSGF CA125 CEA joint detection in the diagnosis of  oophoroma.

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