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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Interferon γ ELISA kit

E11I0345 Bovine Interferon γ ELISA kit

The Bovine Interferon γ ELISA kit can be used to identify samples from the bovine species. Interferon γ can also be called IFIFNγ, IFG, IFI, IFN Gamma, IFNG, IFN-γ, IFN γ.

Specifications of Bovine Interferon γ ELISA kit

Product Information

Cat. No.

E11I0345

Product Name

Bovine Interferon γ ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IFNγ ELISA kit uses an anti-IFNγ antibody and an IFNγ-HRP conjugate in a competitive enzyme immunoassay method. IFNγ-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IFNγ antibody binding site between IFNγ from samples and IFNγ-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IFNγ. Since the number of sites is limited, as more sites are occupied by IFNγ from the sample, fewer sites are left to bind IFNγ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNγ concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Interferon γ ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IFNγ and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Interferon γ ELISA kit

Summary of the Assay Procedure for Bovine Interferon γ ELISA kit

Citations of Bovine Interferon γ ELISA kit

E11I0345 has been referenced in the below publications:

Infection of Cattle With Cryptosporidium parvum: Mast Cell Accumulation in Small Intestine Mucosa.

To separation of lactic acid bacteria and yeast cultures for the application of cow and newborn calf perinatal care.

Cryptosporidium parvum. Protective Immunity against Cryptosporidiosis with Recombinant Lactobacillus Live Vaccines Expressing cp966 after Oral Immunization.

Study on macrophage polarization and cellular immune response against fasciola gigantic and its excretory-secretory products.

Effects of complex anionic salt premix on production performance and health stadus of perinatal cows.

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