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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Interleukin 2 ELISA kit

E12I0308 Chicken Interleukin 2 ELISA kit

Chicken Interleukin 2 ELISA kit is suitable for the detection of samples from Chicken species. Interleukin 2 can also be called Interleukin-2, IL-2, T-cell growth factor, Aldesleukin, Lymphokine, POIL2, T Cell Growth Factor, TCGF, IL2.

Specifications of Chicken Interleukin 2 ELISA kit

Product Information

Cat. No.

E12I0308

Product Name

Chicken Interleukin 2 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL2 antibody and an IL2-HRP conjugate. The assay sample and buffer are incubated together with IL2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL2 concentration since IL2 from samples and IL2-HRP conjugate compete for the anti-IL2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL2 from the sample, fewer sites are left to bind IL2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL2 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Interleukin 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Interleukin 2 ELISA kit

Summary of the Assay Procedure for Chicken Interleukin 2 ELISA kit

Citations of Chicken Interleukin 2 ELISA kit

E12I0308 has been referenced in the below publications:

Protective immunity induced by a DNA vaccine encoding Eimeria tenella rhomboid against homologous challenge.

Screening of Immune Function Related Gene MHC B-LβII and A Certain Purity of Traditional Chinese Medicine Compound Polysaccharides on Immune Regulation in Chicken.

Effects of Xylanase and Polysaccharides from H.caput-medusae (Bun.:Fr) Pers Fermentation Extractum on growth performance, immunization and antioxidation in broilers.


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