Human Cartilage glycoprotein 39/YKL40/Chitinase 3 like Protein 1 ELISA kit

BlueGene Biotech Human Cartilage glycoprotein 39/YKL40/Chitinase 3 like Protein 1 ELISA kit

E01C0072 Human Cartilage glycoprotein 39/YKL40/Chitinase 3 like Protein 1 ELISA kit

The Human Cartilage glycoprotein 39/YKL40/Chitinase 3 like Protein 1 ELISA kit can be used to identify samples from the Human species. Cartilage glycoprotein 39/YKL40/Chitinase 3 like Protein 1 can also be called CHI3L1, 39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP39, chitinase 3 like 1 (cartilage glycoprotein 39), chitinase 3 like 1, Chitinase 3 like protein 1 precursor, chitinase, GP39, HC gp39, HCGP 3P, CGP-39, chitinase 3 like 1, Chitinase-3-like protein 1 hCGP-39, YYL 40.

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Specifications of Human Cartilage glycoprotein 39/YKL40/Chitinase 3 like Protein 1 ELISA kit

Product Information
Cat. No.	E01C0072
Product Name	Human Cartilage glycoprotein 39/YKL40/Chitinase 3 like Protein 1 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5.0 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

YKL40 ELISA kit uses an anti-YKL40 antibody and an YKL40-HRP conjugate in a competitive enzyme immunoassay method. YKL40-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-YKL40 antibody binding site between YKL40 from samples and YKL40-HRP conjugate, the intensity of the color is inversely proportional to the concentration of YKL40. Since the number of sites is limited, as more sites are occupied by YKL40 from the sample, fewer sites are left to bind YKL40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YKL40 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Cartilage glycoprotein 39/YKL40/Chitinase 3 like Protein 1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between YKL40 and analogues was observed.

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Summary of the Assay Procedure for Human Cartilage glycoprotein 39/YKL40/Chitinase 3 like Protein 1 ELISA kit

Citations of Human Cartilage glycoprotein 39/YKL40/Chitinase 3 like Protein 1 ELISA kit

E01C0072 has been referenced in the below publications：

hYKL-40 cancer biomarker electroanalysis in serum samples and model cell lysates: capacitive immunosensing compared with enzyme label immunosorbent assays (ELISA).

Expression and significance of YKL-40 protein in the supernatant of prostate cancer cells.

Change of serum levels of adiponectin and YKL-40 in patients witll different coronary heart diseases and its correlation with the severity of coronary artery lesion.