Name: E.coli Residual Total RNA Detection Kit (qRT-PCR)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech E.coli Residual Total RNA Detection Kit (qRT-PCR)

EC-R100T(100T) E.coli Residual Total RNA Detection Kit (qRT-PCR)

The E.coli Residual Total RNA Detection Kit (qRT-PCR) can be used for quantitative analysis of RNA residue in recombinant protein expressed products, purified intermediate and finished products from host cell.

Products

Specifications of E.coli Residual Total RNA Detection Kit (qRT-PCR)

Introduction

This kit adopts Taqman probe fluorescence qRT-PCR method. The kit has the advantages of high specificity and sensitivity by using specific primers & probes, LOQ can reach 1fg/μL level. This kit can be used in combination with our Magnetic Residual DNA Sample Preparation Kit (Cat#CG-DP050/CG-DP100). The RNA is fully synthetically designed, therefore it has high purity and no protein and ion interference. The RNA exhibits high stability, as confirmed by accelerated degradation testing at 37℃. The kit demonstrates good reproducibility, with consistent results observed among replicate wells within a single assay and across multiple independent experiments.

Kit Components
Components NO.	Components Name	Cat#/Size EC-R100T(100T)
Components NO.	Components Name	Cat#/Size EC-R100T(100T)
B1	2X qRT-PCR Buffer	1mL
B2	qRT-PCR Enzyme Mix	100μL
B3	E.coli Primer & Probe Mix	120μL
B4	E.coli RNA Control (20ng/μL)	50μL
B5	RNase-Free H2O	2*2mL
B6	ROX Reference Dye	40μL
B7	ROX Reference Dye II	40μL
B8	DNase I	20 μL
B9	10 x DNase I Buffer	40μL

Shipping and Storage
1	All components are shipped at -20℃
2	The kit should be stored at -20℃ and it is recommended to be used within one year. B3 should be stored protected from light.

PCR Reaction System on E.coli Residual Total RNA Detection Kit (qRT-PCR)

Components	Volume(μL)
2X qRT-PCR Buffer	10
qRT-PCR Enzyme Mix	1
E.coli Primer & Probe Mix	1.2
RNA template (control or sample)	2
ROX	0.4
RNase-Free H2O	5.4
Total volume	20

Mix solution = (number of reaction wells+4) * (10+1.2+0.4+5.4) μL (including the volume lost in the 4 wells).

Standards and samples are recommended to be tested in triplicate. The detection range of the standard curve mentioned above is suitable for most experiments and can be adjusted as needed.

Criteria for Results

Standard Curve: R²> 0.99; Amplification Efficiency: 90%≤Eff%≤110%; Slope: -3.8~-3.1.

The recovery rate of spiked samples=(measured value of spiked samples - measured value of samples)/theoretical value of spiked samples * 100%, with a range of 50% -150%.
No Template Control (NTC): In the reaction system, replacing the target template with RNase-Free H2O while keeping other components unchanged, and the Ct value should be at least 2 cycles higher than the lowest point of the standard curve.