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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human High Mobility Group box1 Protein ELISA kit

  • Epigenetics and Nuclear Signaling

E01H0009 Human High Mobility Group box1 Protein ELISA kit

The Human High Mobility Group box1 Protein ELISA kit can be used to identify samples from the human species. High Mobility Group box1 Protein can also be called HMGB1, HMG1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein.

Specifications of Human High Mobility Group box1 Protein ELISA kit

Product Information

Cat. No.

E01H0009

Product Name

Human High Mobility Group box1 Protein ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HMGB1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HMGB1. Standards or samples are then added to the microtiter plate wells and HMGB1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HMGB1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HMGB1 are added to each well to “sandwich” the HMGB1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HMGB1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGB1 concentration in each sample is interpolated from this standard curve. 


Quality Control on Human High Mobility Group box1 Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HMGB1 and analogues was observed.


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Summary of the Assay Procedure for Human High Mobility Group box1 Protein ELISA kit

Summary of the Assay Procedure for Human High Mobility Group box1 Protein ELISA kit

Citations of Human High Mobility Group box1 Protein ELISA kit

E01H0009 has been referenced in the below publications:

Elevated HMGB1-related interleukin-6 is associated with dynamic responses of monocytes in patients with active pulmonary tuberculosis.

Baicalin Inhibits -Induced High-Mobility Group Box 1 Release during Inflammation.

The expression of HMGB1 in patients with acute coronary syndrome and  effects of loading dose of atorvastatin in these patients during PCI perioperative period.

Relationship of serum high mobility group protein B1 levels and correlative clinic indexes in sepsis patients.

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