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BlueGene Biotech Human Motor neuron and pancreas homeobox 1 ELISA kit

E01M1092 Human Motor neuron and pancreas homeobox 1 ELISA kit

The Human Motor neuron and pancreas homeobox 1 ELISA kit can be used to identify samples from the human species. Motor neuron and pancreas homeobox 1 can also be called HB9/HLXB9, HB 9, HB9, HLXB 9, HLXB9, Homeo box HB9, Homeobox HB9, Homeobox protein HB9, HOXHB9, MNX1, MNX1_HUMAN, Motor neuron and pancreas homeobox protein 1, Sacral agenesis autosomal dominant (Currarino triad), SCRA 1, SCRA1, SCRA1, HOXHB9.

Specifications of Human Motor neuron and pancreas homeobox 1 ELISA kit

Product Information

Cat. No.

E01M1092

Product Name

Human Motor neuron and pancreas homeobox 1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MNX1 ELISA kit uses an anti-MNX1 antibody and an MNX1-HRP conjugate in a competitive enzyme immunoassay method. MNX1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MNX1 antibody binding site between MNX1 from samples and MNX1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MNX1. Since the number of sites is limited, as more sites are occupied by MNX1 from the sample, fewer sites are left to bind MNX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MNX1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Motor neuron and pancreas homeobox 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MNX1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Motor neuron and pancreas homeobox 1 ELISA kit

Summary of the Assay Procedure for Human Motor neuron and pancreas homeobox 1 ELISA kit

Citations of Human Motor neuron and pancreas homeobox 1 ELISA kit

E01M1092 has been referenced in the below publications:

A study on the role of gene DRD5, MNX1 aberrant methylation in diagnosis of type 2 diabetic macrovascular complications.

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