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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Guinea Pig Histamine ELISA kit (E05H0013)

E05H0013 Guinea Pig Histamine ELISA kit

Guinea Pig Histamine ELISA kit is suitable for the detection of samples from Bovine species. Histamine can also be called Hist, HIS.

Products

Specifications of Guinea Pig Histamine ELISA kit

Product Information

Cat. NO.

E05H0013

Product Name

Guinea Pig Histamine ELISA kit

Species

Guinea Pig

Product Size

48 Tests / 96 Tests

Concentration

25-500 pg/mL

Sensitivity

1.0 pg/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

25 pg/mL

1 vial

STANDARD C (0.5mL)

50 pg/mL

1 vial

STANDARD D (0.5mL)

100 pg/mL

1 vial

STANDARD E (0.5mL)

250 pg/mL

1 vial

STANDARD F (0.5mL)

500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HIS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HIS. Standards or samples are then added to the microtiter plate wells and HIS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HIS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HIS are added to each well to “sandwich” the HIS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HIS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HIS concentration in each sample is interpolated from this standard curve.


Quality Control on Guinea Pig Histamine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-106%

Linearity

Diluent Ratio

Range %

1:2

93-103

1:4

89-108

1:8

86-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HIS and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Guinea Pig Histamine ELISA kit

Summary of the Assay Procedure for Guinea Pig Histamine ELISA kit

Citations of Guinea Pig Histamine ELISA kit

E05H0013 has been referenced in the below publications:

Effects of Selective Inhibition of PDE4 by YM976 on Airway Reactivity and Cough in Ovalbumin-Sensitized Guinea Pigs.

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